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goat polyclonal antibodies against cd31  (R&D Systems)


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    Structured Review

    R&D Systems goat polyclonal antibodies against cd31
    Goat Polyclonal Antibodies Against Cd31, supplied by R&D Systems, used in various techniques. Bioz Stars score: 98/100, based on 1273 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+antibody+against+cd31/Human%2FMouse%2FRat+CD31%2FPECAM-1+Antibody/pm41665021-283-8-13
    Average 98 stars, based on 1273 article reviews
    goat polyclonal antibodies against cd31 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Persistent Activation of Endothelial Cells is Linked to Thrombosis and Inflammation in Cerebral Cavernous Malformation Disease
    Article Snippet: Cell nuclei were stained and mounted using DAPI Fluoromount-G mounting medium (SouthernBiotech, #0100). .. Human CCM tissue was similarly processed for immunofluorescence analysis, and sections were incubated in rabbit monoclonal antibodies against JUNB (1:200, #3753S; Cell Signaling), goat polyclonal antibody against CD31 (1:100, #AF3628; R&D Systems) in PBS at room temperature. .. Tissue sections were washed four times in PBS and incubated, with Alexa Fluor 488 anti-rabbit secondary antibodies (1:300, #711-546-152; Jackson Laboratory) and Alexa Fluor 594 anti-goat secondary antibodies (1:300, #705-585-003; Jackson Laboratory) in PBS for 2h at RT.

    Incubation:

    Article Title: Persistent Activation of Endothelial Cells is Linked to Thrombosis and Inflammation in Cerebral Cavernous Malformation Disease
    Article Snippet: Cell nuclei were stained and mounted using DAPI Fluoromount-G mounting medium (SouthernBiotech, #0100). .. Human CCM tissue was similarly processed for immunofluorescence analysis, and sections were incubated in rabbit monoclonal antibodies against JUNB (1:200, #3753S; Cell Signaling), goat polyclonal antibody against CD31 (1:100, #AF3628; R&D Systems) in PBS at room temperature. .. Tissue sections were washed four times in PBS and incubated, with Alexa Fluor 488 anti-rabbit secondary antibodies (1:300, #711-546-152; Jackson Laboratory) and Alexa Fluor 594 anti-goat secondary antibodies (1:300, #705-585-003; Jackson Laboratory) in PBS for 2h at RT.

    Bioprocessing:

    Article Title: Persistent Activation of Endothelial Cells is Linked to Thrombosis and Inflammation in Cerebral Cavernous Malformation Disease
    Article Snippet: Cell nuclei were stained and mounted using DAPI Fluoromount-G mounting medium (SouthernBiotech, #0100). .. Human CCM tissue was similarly processed for immunofluorescence analysis, and sections were incubated in rabbit monoclonal antibodies against JUNB (1:200, #3753S; Cell Signaling), goat polyclonal antibody against CD31 (1:100, #AF3628; R&D Systems) in PBS at room temperature. .. Tissue sections were washed four times in PBS and incubated, with Alexa Fluor 488 anti-rabbit secondary antibodies (1:300, #711-546-152; Jackson Laboratory) and Alexa Fluor 594 anti-goat secondary antibodies (1:300, #705-585-003; Jackson Laboratory) in PBS for 2h at RT.



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    A) HOMER motif enrichment analysis showing -log 10 p -values for putative enhancers with increased accessibility in Pdcd10 BECKO . B) Junb expression and regulatory activity across endothelial subtypes. Top: Junb expression in Pdcd10 BECKO (pink dots) and Pdcd10 fl/fl (blue dots) across endothelial subtypes. Each point represents a biological replicate. FDR values were calculated using edgeR’s glmLRT function; ns, not significant. Middle: Expression of JUNB-regulated differentially expressed genes (DEGs) (n = 2,029 out of 3262) across BEC subtypes. Each point represents one DEG. Bottom: Chromatin accessibility of JUNB motif-containing putative enhancers linked to DEGs (n = 5124), per BEC subtype. Each point represents an individual putative enhancer. For panels (Middle) and (Bottom) , P -values from Wilcoxon rank-sum tests. For the top panel, error bars represent mean ± SEM. Subtype-specific colors indicate the endothelial subtype. C) Immunofluorescence staining in Pdcd10 BECKO -tdtTomato mice showing colocalization of JUNB (green) with CCM endothelial cells (tdTomato, red), both delimiting and within the CCM lesion. Arrows indicate representative JUNB-positive endothelial cells. Asterisk marks lumen of CCM lesion. Nuclei are stained with DAPI (blue). D) Immunofluorescence staining of human CCM lesion showing JUNB-positive (green) CCM endothelial cells stained with <t>CD31</t> (red). JUNB expression is observed both in the surrounding area and within the CCM lesion. Arrows point to representative JUNB-positive BECs. Nuclei are stained with DAPI (blue).
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    A) HOMER motif enrichment analysis showing -log 10 p -values for putative enhancers with increased accessibility in Pdcd10 BECKO . B) Junb expression and regulatory activity across endothelial subtypes. Top: Junb expression in Pdcd10 BECKO (pink dots) and Pdcd10 fl/fl (blue dots) across endothelial subtypes. Each point represents a biological replicate. FDR values were calculated using edgeR’s glmLRT function; ns, not significant. Middle: Expression of JUNB-regulated differentially expressed genes (DEGs) (n = 2,029 out of 3262) across BEC subtypes. Each point represents one DEG. Bottom: Chromatin accessibility of JUNB motif-containing putative enhancers linked to DEGs (n = 5124), per BEC subtype. Each point represents an individual putative enhancer. For panels (Middle) and (Bottom) , P -values from Wilcoxon rank-sum tests. For the top panel, error bars represent mean ± SEM. Subtype-specific colors indicate the endothelial subtype. C) Immunofluorescence staining in Pdcd10 BECKO -tdtTomato mice showing colocalization of JUNB (green) with CCM endothelial cells (tdTomato, red), both delimiting and within the CCM lesion. Arrows indicate representative JUNB-positive endothelial cells. Asterisk marks lumen of CCM lesion. Nuclei are stained with DAPI (blue). D) Immunofluorescence staining of human CCM lesion showing JUNB-positive (green) CCM endothelial cells stained with <t>CD31</t> (red). JUNB expression is observed both in the surrounding area and within the CCM lesion. Arrows point to representative JUNB-positive BECs. Nuclei are stained with DAPI (blue).
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    A) HOMER motif enrichment analysis showing -log 10 p -values for putative enhancers with increased accessibility in Pdcd10 BECKO . B) Junb expression and regulatory activity across endothelial subtypes. Top: Junb expression in Pdcd10 BECKO (pink dots) and Pdcd10 fl/fl (blue dots) across endothelial subtypes. Each point represents a biological replicate. FDR values were calculated using edgeR’s glmLRT function; ns, not significant. Middle: Expression of JUNB-regulated differentially expressed genes (DEGs) (n = 2,029 out of 3262) across BEC subtypes. Each point represents one DEG. Bottom: Chromatin accessibility of JUNB motif-containing putative enhancers linked to DEGs (n = 5124), per BEC subtype. Each point represents an individual putative enhancer. For panels (Middle) and (Bottom) , P -values from Wilcoxon rank-sum tests. For the top panel, error bars represent mean ± SEM. Subtype-specific colors indicate the endothelial subtype. C) Immunofluorescence staining in Pdcd10 BECKO -tdtTomato mice showing colocalization of JUNB (green) with CCM endothelial cells (tdTomato, red), both delimiting and within the CCM lesion. Arrows indicate representative JUNB-positive endothelial cells. Asterisk marks lumen of CCM lesion. Nuclei are stained with DAPI (blue). D) Immunofluorescence staining of human CCM lesion showing JUNB-positive (green) CCM endothelial cells stained with <t>CD31</t> (red). JUNB expression is observed both in the surrounding area and within the CCM lesion. Arrows point to representative JUNB-positive BECs. Nuclei are stained with DAPI (blue).
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    Image Search Results


    A) HOMER motif enrichment analysis showing -log 10 p -values for putative enhancers with increased accessibility in Pdcd10 BECKO . B) Junb expression and regulatory activity across endothelial subtypes. Top: Junb expression in Pdcd10 BECKO (pink dots) and Pdcd10 fl/fl (blue dots) across endothelial subtypes. Each point represents a biological replicate. FDR values were calculated using edgeR’s glmLRT function; ns, not significant. Middle: Expression of JUNB-regulated differentially expressed genes (DEGs) (n = 2,029 out of 3262) across BEC subtypes. Each point represents one DEG. Bottom: Chromatin accessibility of JUNB motif-containing putative enhancers linked to DEGs (n = 5124), per BEC subtype. Each point represents an individual putative enhancer. For panels (Middle) and (Bottom) , P -values from Wilcoxon rank-sum tests. For the top panel, error bars represent mean ± SEM. Subtype-specific colors indicate the endothelial subtype. C) Immunofluorescence staining in Pdcd10 BECKO -tdtTomato mice showing colocalization of JUNB (green) with CCM endothelial cells (tdTomato, red), both delimiting and within the CCM lesion. Arrows indicate representative JUNB-positive endothelial cells. Asterisk marks lumen of CCM lesion. Nuclei are stained with DAPI (blue). D) Immunofluorescence staining of human CCM lesion showing JUNB-positive (green) CCM endothelial cells stained with CD31 (red). JUNB expression is observed both in the surrounding area and within the CCM lesion. Arrows point to representative JUNB-positive BECs. Nuclei are stained with DAPI (blue).

    Journal: bioRxiv

    Article Title: Persistent Activation of Endothelial Cells is Linked to Thrombosis and Inflammation in Cerebral Cavernous Malformation Disease

    doi: 10.1101/2025.06.29.662238

    Figure Lengend Snippet: A) HOMER motif enrichment analysis showing -log 10 p -values for putative enhancers with increased accessibility in Pdcd10 BECKO . B) Junb expression and regulatory activity across endothelial subtypes. Top: Junb expression in Pdcd10 BECKO (pink dots) and Pdcd10 fl/fl (blue dots) across endothelial subtypes. Each point represents a biological replicate. FDR values were calculated using edgeR’s glmLRT function; ns, not significant. Middle: Expression of JUNB-regulated differentially expressed genes (DEGs) (n = 2,029 out of 3262) across BEC subtypes. Each point represents one DEG. Bottom: Chromatin accessibility of JUNB motif-containing putative enhancers linked to DEGs (n = 5124), per BEC subtype. Each point represents an individual putative enhancer. For panels (Middle) and (Bottom) , P -values from Wilcoxon rank-sum tests. For the top panel, error bars represent mean ± SEM. Subtype-specific colors indicate the endothelial subtype. C) Immunofluorescence staining in Pdcd10 BECKO -tdtTomato mice showing colocalization of JUNB (green) with CCM endothelial cells (tdTomato, red), both delimiting and within the CCM lesion. Arrows indicate representative JUNB-positive endothelial cells. Asterisk marks lumen of CCM lesion. Nuclei are stained with DAPI (blue). D) Immunofluorescence staining of human CCM lesion showing JUNB-positive (green) CCM endothelial cells stained with CD31 (red). JUNB expression is observed both in the surrounding area and within the CCM lesion. Arrows point to representative JUNB-positive BECs. Nuclei are stained with DAPI (blue).

    Article Snippet: Human CCM tissue was similarly processed for immunofluorescence analysis, and sections were incubated in rabbit monoclonal antibodies against JUNB (1:200, #3753S; Cell Signaling), goat polyclonal antibody against CD31 (1:100, #AF3628; R&D Systems) in PBS at room temperature.

    Techniques: Expressing, Activity Assay, Immunofluorescence, Staining